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Home >> Antibodies >> Recombinant EGFRvIII Antibody / EGF Receptor Variant III

Recombinant EGFRvIII Antibody / EGF Receptor Variant III [clone GFR/2600R] (V3980)

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  Catalog No Formulation Size Price (USD)  
Image V3980-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3980-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3980SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3980IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC testing of FFPE human lung squamous cell carcinoma with recombinant EGFRvIII antibody (clone GFR/2600R). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.
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IHC testing of FFPE human lung squamous cell carcinoma with recombinant EGFRvIII antibody (clone GFR/2600R). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.
IHC testing of FFPE human placenta with recombinant EGFRvIII antibody (clone GFR/2600R). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.
SDS-PAGE analysis of purified, BSA-free recombinant EGFRvIII antibody (clone GFR/2600R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human. Other species not tested.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name GFR/2600R
Purity Protein A affinity chromatography
UniProt P00533
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant EGFRvIII antibody is available for research use only.
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Description

EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Application Notes

Titering of the recombinant EGFRvIII antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids LEEKKGNYVVTDHC were used as the immunogen for the recombinant EGFRvIII antibody. This is the predicted sequence of the exon 2-7 delete fusion region.

Storage

Store the recombinant EGFRvIII antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all EGFRvIII antibodies

   2.   View all recombinant antibodies

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