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Home >> Antibodies >> Recombinant EGFR Antibody L858R Mutant Specific

Recombinant EGFR Antibody L858R Mutant Specific [clone GFR/4564R] (V8746)

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  Catalog No Formulation Size Price (USD)  
Image V8746-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8746-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8746SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human placenta with recombinant EGFR antibody (clone GFR/4564R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placenta with recombinant EGFR antibody (clone GFR/4564R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta with recombinant EGFR antibody (clone GFR/4564R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name GFR/4564R
Purity Protein A affinity chromatography
UniProt P00533
Localization Cytomembrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant EGFR antibody is available for research use only.
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Description

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity). The epitope for this antibody is specifically expressed on EGFR-L858R mutated lung cancer.

Application Notes

Optimal dilution of the recombinant EGFR antibody should be determined by the researcher.

Immunogen

A portion of amino acids 800-900 of EGF receptor (mutated L858R) was used as the immunogen for the recombinant EGFR antibody.

Storage

Store the recombinant EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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