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Home >> Antibodies >> Recombinant EGF Receptor / EGFR Antibody (Extracellular domain)

Recombinant EGF Receptor / EGFR Antibody (Extracellular domain) [clone rEGFR/6389] (V9273)

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  Catalog No Formulation Size Price (USD)  
Image V9273-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9273-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9273SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human placental tissue with recombinant EGF Receptor antibody (clone rEGFR/6389). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placental tissue with recombinant EGF Receptor antibody (clone rEGFR/6389). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human testis tissue with recombinant EGF Receptor antibody (clone rEGFR/6389) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant EGF Receptor antibody (clone rEGFR/6389) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name rEGFR/6389
Purity Protein A/G affinity
UniProt P00533
Localization Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant EGF Receptor antibody is available for research use only.
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Description

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Application Notes

Optimal dilution of the recombinant EGF Receptor antibody should be determined by the researcher.

Immunogen

Amino acids 25-645 from the extracellular domain of the human protein were used as the immunogen for the recombinant EGF Receptor antibody.

Storage

Aliquot the recombinant EGF Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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