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Home >> Antibodies >> Recombinant EGF Receptor Antibody

Recombinant EGF Receptor Antibody [clone GFR/2968R] (V3981)

  Catalog No Formulation Size Price (USD)  
Image V3981-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3981-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3981SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3981IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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Western blot testing of human A431 cell lysate with recombinant EGF Receptor antibody. Expected molecular weight: 134-180 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free recombinant EGF Receptor antibody (clone GFR/2968R) as confirmation of integrity and purity.
IHC testing of FFPE human placenta with recombinant EGF Receptor antibody (clone GFR/2968R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant EGF Receptor antibody (clone GFR/2968R). These results demonstrate the foremost specificity of the GFR/2968R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name GFR/2968R
Purity Protein A affinity chromatography
UniProt P00533
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant EGF Receptor antibody is available for research use only.
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Description

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. [UniProt]

Application Notes

Titering of the recombinant EGF Receptor antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the recombinant EGF Receptor antibody.

Storage

Store the recombinant EGF Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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