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Home >> Antibodies >> Recombinant E-Cadherin Antibody

Recombinant E-Cadherin Antibody [clone RM244] (R20265)

  Catalog No Formulation Size Price (USD)  
Image R20265-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot of human MCF7 lysate using recombinant E-Cadherin antibody at 1:1000. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
IHC testing of FFPE human breast cancer tissue with recombinant E-Cadherin antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM244
Purity Protein A purified from animal origin-free supernatant
UniProt P12830
Gene ID 999
Localization Membrane, cytoplasmic
Applications Immunohistochemistry : 1:500-1:1000 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant E-Cadherin antibody is available for research use only.
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Description

This recombinant E-Cadherin antibody reacts to human E-cadherin/Cadherin-/CDH1.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant E-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to E-Cadherin/CDH1 was used as the immunogen for this recombinant E-Cadherin antibody.

Storage

Store the recombinant E-Cadherin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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