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Home >> Antibodies >> Recombinant E-Cadherin Antibody / Rabbit Monoclonal

Recombinant E-Cadherin Antibody / Rabbit Monoclonal [clone ECD1-3R] (V3702)

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  Catalog No Formulation Size Price (USD)  
Image V3702-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3702-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3702SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3702IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone ECD1-3R, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone ECD1-3R, green) and Reddot nuclear stain (red).
Western blot testing of human MCF-7 cell lysate with recombinant E-Cadherin antibody (clone ECD1-3R). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
Formalin-fixed, paraffin-embedded human cervix stained with recombinant E-Cadherin antibody (clone ECD1-3R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human MCF7 cells with recombinant E-Cadherin antibody (clone ECD1-3R); Red=isotype control, Blue= recombinant E-Cadherin antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ECD1-3R
Purity Protein A affinity chromatography
UniProt P12830
Localization Cytoplasmic, membranous
Applications Western blot : 1-2ug/ml
Immunofluorescence : 1-2ug/ml
Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant E-Cadherin antibody is available for research use only.
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Description

Cadherins behave as both receptors and ligands for other molecules. During development, their behavior assists in properly positioning cells: they are responsible for the separation of the different tissue layers, and for cellular migration. In the very early stages of development, E-cadherin (epithelial cadherin) is most greatly expressed. The E-cadherin-catenin complex plays a key role in cellular adhesion; loss of this function has been associated with greater tumour metastasis. [Wiki]

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant E-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the recombinant E-Cadherin antibody.

Storage

Store the recombinant E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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