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Home >> Antibodies >> Recombinant dsDNA Antibody

Recombinant dsDNA Antibody [clone rDSD/4565] (V8639)

  Catalog No Formulation Size Price (USD)  
Image V8639-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8639-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8639SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human colon with recombinant dsDNA antibody (clone rDSD/4565). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node with recombinant dsDNA antibody (clone rDSD/4565). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant dsDNA antibody (clone rDSD/4565) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name rDSD/4565
Purity Protein G affinity chromatography
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant dsDNA antibody is available for research use only.
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Description

This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Application Notes

Optimal dilution of the recombinant dsDNA antibody should be determined by the researcher.

Immunogen

Nuclei of Burkitt cells were used as the immunogen for the recombinant dsDNA antibody.

Storage

Store the recombinant dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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