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Home >> Antibodies >> Recombinant Desmin Antibody

Recombinant Desmin Antibody [clone rDES/1711] (V9768)

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  Catalog No Formulation Size Price (USD)  
Image V9768-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9768-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9768SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC testing of FFPE human uterus with recombinant Desmin antibody (clone rDES/1711). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human uterus with recombinant Desmin antibody (clone rDES/1711). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human uterus with recombinant Desmin antibody (clone rDES/1711). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free recombinant Desmin Antibody (clone rDES/1711). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rDES/1711
Purity Protein G affinity chromatography
UniProt P17661
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant Desmin antibody is available for research use only.
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Description

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Application Notes

Optimal dilution of the recombinant Desmin antibody should be determined by the researcher.

Immunogen

Recombinant human protein was used as the immunogen for the recombinant Desmin antibody.

Storage

Store the recombinant Desmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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