deltaNp63 Antibody / Epithelial Cell Identity and Lineage Stability Marker Antibody [clone P40/4396R] (V9359)

deltaNp63 Antibody. Immunohistochemistry analysis of FFPE human prostate tissue demonstrates strong HRP-DAB brown nuclear staining in basal epithelial cells outlining glandular structures, while luminal secretory cells remain negative. The nuclear-restricted pattern highlights epithelial cell populations and supports use of this antibody as an epithelial identity regulator, with deltaNp63 expression reflecting TP63-driven maintenance of epithelial lineage. Staining is crisp with minimal background, allowing clear visualization of gland architecture and basal cell compartmentalization. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human prostate tissue with recombinant deltaNp63 antibody (clone P40/4396R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

deltaNp63 Antibody. Flow cytometry analysis of PFA-fixed and permeabilized human HeLa cells demonstrates a rightward shift in fluorescence intensity for the deltaNp63-stained population (blue) compared to the isotype control (red), indicating specific intracellular detection of Tumor protein p40 (TP63). The separation between populations supports accurate gating and quantification of deltaNp63-positive cells, consistent with nuclear expression of this transcription factor following permeabilization. The staining pattern supports use of this antibody as an epithelial identity regulator, reflecting TP63-driven maintenance of epithelial lineage at the single-cell population level.