Decorin Antibody Recombinant Rabbit MAb / DCN [clone DCN/8715R] (V4314)

Western blot analysis of Decorin antibody in human tissues. Lysates from human heart, human lung, and human skeletal muscle were probed using recombinant rabbit monoclonal Decorin antibody (clone DCN/8715R). A prominent immunoreactive band is observed at approximately 90-100 kDa, consistent with the glycosylated form of Decorin. The predicted molecular weight of the Decorin core protein is approximately 40 kDa; however, Decorin is a proteoglycan that carries glycosaminoglycan side chains, resulting in a higher apparent molecular weight on SDS-PAGE. The banding pattern across multiple tissue types is consistent with extracellular matrix-associated Decorin expression.

Western blot analysis of Decorin antibody in multi-species heart and lung tissues. Lysates from human heart, mouse heart, rat heart, hamster heart, guinea pig heart, rabbit heart, as well as human lung, mouse lung, rat lung, hamster lung, guinea pig lung, and rabbit lung were probed using recombinant rabbit monoclonal Decorin antibody (clone DCN/8715R). A prominent immunoreactive band is detected at approximately 90-100 kDa across species, consistent with the glycosylated form of Decorin.
The predicted molecular weight of the Decorin core protein is approximately 40 kDa; however, Decorin is a small leucine-rich proteoglycan that carries glycosaminoglycan side chains, resulting in a higher apparent molecular weight on SDS-PAGE. The conserved banding pattern across multiple species supports cross-species recognition of Decorin and is consistent with its extracellular matrix localization in connective tissues.

Immunohistochemistry of Decorin antibody in human prostate carcinoma tissue. Formalin-fixed, paraffin-embedded human prostate carcinoma was stained using recombinant rabbit monoclonal Decorin antibody (clone DCN/8715R). Heat induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 20 min followed by cooling prior to staining. HRP-DAB brown chromogenic signal highlights extracellular stromal connective tissue surrounding malignant glands, consistent with Decorin localization within collagen-rich matrix compartments.

SDS-PAGE analysis of purified, BSA-free Decorin antibody recombinant rabbit mAb clone DCN/8715R as confirmation of integrity and purity.