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Home >> Antibodies >> Recombinant Cytokeratin 7 Antibody

Recombinant Cytokeratin 7 Antibody [clone rOV-TL12/30] (V3706)

  Catalog No Formulation Size Price (USD)  
Image V3706-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3706-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3706SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3706IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recmousemono
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IHC testing of FFPE human endometrial carcinoma with recombinant Cytokeratin 7 antibody (clone rOV-TL12/30). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 7 antibody (clone rOV-TL12/30) as confirmation of integrity and purity.
Western blot testing of human HeLa cell lysate with recombinant Cytokeratin 7 antibody (clone rOV-TL12/30).
Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Cytokeratin 7 antibody (clone rOV-TL12/30); Red=isotype control, Blue= recombinant Cytokeratin 7 antibody.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant Cytokeratin 7 antibody (clone rOV-TL12/30). These results demonstrate the foremost specificity of the rOV-TL12/30 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rOV-TL12/30
Purity Protein G affinity chromatography
UniProt P08729
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-2ug/million cells
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant Cytokeratin 7 antibody is available for research use only.
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Description

CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt]

Application Notes

The stated application concentrations are suggested starting amounts. Optimal dilution of the recombinant Cytokeratin 7 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the recombinant Cytokeratin 7 antibody.

Storage

Store the recombinant Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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