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Home >> Antibodies >> Recombinant Cytokeratin 7 Antibody / Rabbit Monoclonal

Recombinant Cytokeratin 7 Antibody / Rabbit Monoclonal [clone KRT7/1499R] (V7258)

  Catalog No Formulation Size Price (USD)  
Image V7258-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V7258-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V7258SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V7258IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of FFPE human oviduct with recombinant Cytokeratin 7 antibody (clone KRT7/1499R). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Western blot testing of human HeLa cell lysate with recombinant Cytokeratin 7 antibody (clone KRT7/1499R). Predicted molecular weight ~51 kDa.
Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Cytokeratin 7 antibody (clone KRT7/1499R); Red=isotype control, Blue= recombinant Cytokeratin 7 antibody.
SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 7 antibody (clone KRT7/1499R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name KRT7/1499R
Purity Protein A affinity chromatography
UniProt P08729
Localization Cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Western blot : 0.5-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant Cytokeratin 7 antibody is available for research use only.
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Description

Cytokeratin-7 (CK7) is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK7 is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. It is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain CK7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the protein is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively. It is commonly used together with CK20 when making such diagnoses. [Wiki]

Application Notes

Optimal dilution of the recombinant Cytokeratin 7 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full-length human CK7 protein was used as the immunogen for the recombinant Cytokeratin 7 antibody.

Storage

Store the recombinant Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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