KRT19 Antibody rKRT19/799 / Recombinant Cytokeratin 19 Monoclonal Antibody [clone rKRT19/799] (V3614)

KRT19 Antibody rKRT19/799 by IHC in human colon tissue. Immunohistochemistry of formalin-fixed, paraffin-embedded human colon tissue using recombinant KRT19 Antibody clone rKRT19/799. Brown cytoplasmic immunoreactivity highlights epithelial cells lining the intestinal glandular structures, with staining following the elongated crypt architecture. The surrounding connective tissue exhibits comparatively limited staining, while hematoxylin counterstaining reveals nuclei in blue. The Recombinant Cytokeratin 19 Monoclonal Antibody demonstrates the distribution of KRT19 within colonic epithelial cells. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

KRT19 Antibody rKRT19/799 by FACS in human MCF7 cells. Flow cytometric analysis of paraformaldehyde-fixed human MCF7 breast carcinoma cells using recombinant KRT19 Antibody clone rKRT19/799. The blue histogram represents antibody-stained cells, while the red histogram represents the isotype control. The antibody-stained population exhibits a pronounced rightward fluorescence shift, with its peak approximately one logarithmic unit above the control population. Although the distributions overlap at their margins, the substantial separation of their principal peaks demonstrates increased antibody-associated fluorescence in the tested cells. The Recombinant Cytokeratin 19 Monoclonal Antibody detects KRT19-associated fluorescence in this human epithelial carcinoma cell model.

KRT19 Antibody rKRT19/799 by WB in human HepG2 cells. Western blot analysis of human HepG2 hepatocellular carcinoma cell lysate using recombinant KRT19 Antibody clone rKRT19/799. The blot displays a single prominent immunoreactive band migrating between the 37 and 50 kDa molecular weight markers, at approximately 43-45 kDa. This position is consistent with the predicted molecular weight of human cytokeratin 19, approximately 43 kDa. No prominent additional bands are visible within the displayed molecular weight range. The relatively clean background and concentrated signal demonstrate recognition of a protein migrating near the expected size of KRT19. The Recombinant Cytokeratin 19 Monoclonal Antibody supports detection of KRT19 in human HepG2 cell lysate under the tested conditions.

KRT19 Antibody rKRT19/799 by human protein microarray specificity analysis. Protein microarray analysis using recombinant KRT19 Antibody clone rKRT19/799 against a HuProt array containing more than 19,000 full-length human proteins. The graph shows a dominant signal at the highest-ranked protein, followed by substantially weaker signals among the remaining ranked proteins. The accompanying table identifies KRT19 as the leading target, with markedly lower Z-scores for the next-ranked proteins. Z-scores represent signal strength relative to the overall array distribution, while S-scores describe the separation between ranked signals. The results support preferential recognition of KRT19 within the tested protein array and provide protein microarray validation of antibody specificity.

SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 19 antibody (clone rKRT19/799) as confirmation of integrity and purity.