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Home >> Antibodies >> Recombinant Cytokeratin 19 Antibody / Rabbit Monoclonal

Recombinant Cytokeratin 19 Antibody / Rabbit Monoclonal [clone CTKN19-2R] (V3743)

  Catalog No Formulation Size Price (USD)  
Image V3743-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3743-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3743SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3743IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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Western blot testing of human HepG2 antibody with Cytokeratin 19 antibody (clone CTKN19-2R). Predicted molecular weight ~43 kDa.
IHC testing of FFPE human colon tissue with recombinant Cytokeratin 19 antibody (clone CTKN19-2R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CTKN19-2R
Purity Protein A affinity chromatography
UniProt P08727
Localization Cytoplasmic, cell membrane, secreted
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant Cytokeratin 19 antibody is available for research use only.
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Description

Keratin 19 is a type I keratin. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelops the developing epidermis. [Wiki]

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Cytokeratin 19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human mammary epithelial organoids were used as the immunogen for the recombinant Cytokeratin 19 antibody.

Storage

Store the recombinant Cytokeratin 19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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