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Home >> Antibodies >> Recombinant Cytokeratin 18 Antibody

Recombinant Cytokeratin 18 Antibody [clone RM279] (R20296)

  Catalog No Formulation Size Price (USD)  
Image R20296-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot of human MCF7 and HeLa cell lysate using recombinant Cytokeratin 18 antibody at 1:1000. Predicted molecular weight ~48 kDa.
IHC testing of FFPE human breast cancer tissue with recombinant Cytokeratin 18 antibody at 1:4000.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM279
Purity Protein A purified from animal origin-free supernatant
UniProt P05783
Gene ID 3875
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:1000-1:4000 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant Cytokeratin 18 antibody is available for research use only.
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Description

This recombinant Cytokeratin 18 antibody reacts to human CK18/Cytokeratin-18.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Cytokeratin 18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the N-terminus of human Cytokeratin 18 was used as the immunogen for this recombinant Cytokeratin 18 antibody.

Storage

Store the recombinant Cytokeratin 18 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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