KRT18 Antibody KRT18/2819R / Keratin 18 Rabbit Monoclonal Antibody [clone KRT18/2819R] (V7299)

KRT18 Antibody KRT18/2819R by IF in human MCF7 cells. Immunofluorescence staining of permeabilized human MCF7 breast carcinoma cells using KRT18 Antibody clone KRT18/2819R, detected in cyan-blue, with RedDot nuclear counterstaining in red. Cytoplasmic fluorescence highlights a filamentous network surrounding the nuclei and extending toward the cell periphery. The staining is particularly prominent along portions of the cytoplasmic margins and within interconnected filament structures. Red nuclear staining provides contrast with the surrounding cytoplasmic signal. The Keratin 18 Rabbit Monoclonal Antibody demonstrates the characteristic cytoplasmic organization of KRT18 intermediate filaments in this human epithelial carcinoma cell model.

KRT18 Antibody KRT18/2819R by IHC in human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human colon carcinoma tissue using KRT18 Antibody clone KRT18/2819R. Strong brown cytoplasmic immunoreactivity highlights numerous neoplastic glandular epithelial cells arranged around irregular luminal spaces. The staining is widespread throughout the carcinoma epithelium, while the surrounding stromal tissue exhibits comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The Keratin 18 Rabbit Monoclonal Antibody demonstrates KRT18 expression within the glandular epithelial compartment of colorectal carcinoma. Heat-induced epitope retrieval was performed using 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling before antibody incubation.

KRT18 Antibody KRT18/2819R by WB in human HCT116 cells. Western blot analysis of human HCT116 colorectal carcinoma cell lysate using KRT18 Antibody clone KRT18/2819R. A single prominent immunoreactive band is detected at approximately 42 kDa, migrating below the predicted molecular weight of human KRT18, approximately 48 kDa. Molecular weight markers are displayed alongside the sample lane, with the detected band positioned between the 37 and 50 kDa markers. The blot exhibits relatively low background without prominent additional bands. Although KRT18 can undergo proteolytic processing, the identity of this lower-migrating band has not been established. The Keratin 18 Rabbit Monoclonal Antibody demonstrates detectable immunoreactivity in HCT116 cell lysate.

KRT18 Antibody KRT18/2819R by FACS in human HeLa cells. Flow cytometric analysis of paraformaldehyde-fixed human HeLa cells using KRT18 Antibody clone KRT18/2819R. The cyan-blue histogram represents antibody-stained cells, while the red histogram represents the isotype control. The antibody-stained population exhibits a pronounced rightward fluorescence shift relative to the control, with its principal peak occurring at substantially higher fluorescence intensity. The two distributions show limited overlap, indicating clear separation between antibody-associated fluorescence and control staining. The Keratin 18 Rabbit Monoclonal Antibody demonstrates detection of KRT18-associated fluorescence in fixed human HeLa cells under the tested conditions.

SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 18 antibody (clone KRT18/2819R) as confirmation of integrity and purity.

Western Blot Analysis of Human Intestine, Human Liver, Human Lung, A431, and HeLa lysates using KRT18 Antibody KRT18/2819R.