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Home >> Antibodies >> Recombinant Cytokeratin 16 Antibody / Rabbit Monoclonal

Recombinant Cytokeratin 16 Antibody / Rabbit Monoclonal [clone CTKN16-1R] (V3737)

  Catalog No Formulation Size Price (USD)  
Image V3737-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3737-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3737SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3737IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC testing of FFPE human skin with recombinant Cytokeratin 16 antibody (clone CTKN16-1R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CTKN16-1R
Purity Protein A affinity chromatography
UniProt P08779
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant Cytokeratin 16 antibody is available for research use only.
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Description

Cytokeratin 16 is an epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Cytokeratin 16 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A C-terminal region peptide was used as the immunogen for the recombinant Cytokeratin 16 antibody.

Storage

Store the recombinant Cytokeratin 16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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