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Home >> Antibodies >> Cytokeratin 14 Antibody KRT14/7054R / CD14 Recombinant Rabbit MAb

Cytokeratin 14 Antibody KRT14/7054R / CD14 Recombinant Rabbit MAb [clone KRT14/7054R] (V4487)

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  Catalog No Formulation Size Price (USD)  
Image V4487-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4487-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4487SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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Cytokeratin 14 Antibody KRT14/7054R Immunohistochemistry Analysis. IHC staining of FFPE human prostate tissue with recombinant Cytokeratin 14 antibody (clone KRT14/7054R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 14 antibody (clone KRT14/7054R) as confirmation of integrity and purity.
Western blot testing of human A431 cell lysate with recombinant Cytokeratin 14 antibody (clone KRT14/7054R). Predicted molecular weight ~53 kDa.
Western blot testing of human skin tissue lysate with Cytokeratin 14 Antibody KRT14/7054R. Predicted molecular weight ~53 kDa.
Western blot analysis of skin tissue lysates of different species using Cytokeratin 14 Antibody KRT14/7054R.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name KRT14/7054R
Purity Protein A/G affinity
UniProt P02533
Localization Cytoplasm
Applications Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This Cytokeratin 14 Antibody KRT14/7054R is available for research use only.
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Description

Cytokeratin 14 Antibody KRT14/7054R detect KRT14 protein, a member of the type I keratin family of intermediate filament proteins. It always pairs with the type II keratin K5 and form the primary keratin pair of the keratinocytes of stratified squamous epithelia, including the epidermis as well as mucosal non-keratinizing stratified squamous epithelia. Cytokeratin 14 is strongly expressed in the undifferentiated basal cell layer containing the stem cells and are down-regulated in the differentiating suprabasal cell layers.

Explore our KRT14 Antibody KRT14/4128 for investigating cytokeratin 14 expression, basal epithelial differentiation, and intermediate filament organization in normal and diseased tissues.

Application Notes

Optimal dilution of the Cytokeratin 14 Antibody KRT14/7054R should be determined by the researcher.

Immunogen

A recombinant human KRT14 fragment (within amino acids 350-472) was used as the immunogen for the recombinant Cytokeratin 14 antibody.

Storage

Aliquot the Cytokeratin 14 Antibody KRT14/7054R and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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