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Home >> Antibodies >> Recombinant Cystic Fibrosis Transmembrane Regulator Antibody / CFTR

Recombinant Cystic Fibrosis Transmembrane Regulator Antibody / CFTR [clone CFTR/7154R] (V9549)

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  Catalog No Formulation Size Price (USD)  
Image V9549-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9549-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9549SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human salivary gland with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human salivary gland with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreas tissue with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung carcinoma tissue with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with recombinant Cystic Fibrosis Transmembrane Regulator antibody (clone CFTR/7154R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CFTR/7154R
Purity Protein A/G affinity
UniProt P13569
Localization Cell surface, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Cystic Fibrosis Transmembrane Regulator antibody is available for research use only.
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Description

Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Application Notes

Optimal dilution of the recombinant Cystic Fibrosis Transmembrane Regulator antibody should be determined by the researcher.

Immunogen

A portion of amino acids 258-385 was used as the immunogen for the recombinant Cystic Fibrosis Transmembrane Regulator antibody.

Storage

Aliquot the recombinant Cystic Fibrosis Transmembrane Regulator antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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