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Home >> Antibodies >> Recombinant Cyclin E1 Antibody / CCNE1

Recombinant Cyclin E1 Antibody / CCNE1 [clone rCCNE1/4936] (V9151)

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  Catalog No Formulation Size Price (USD)  
Image V9151-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9151-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9151SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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Western blot testing of human HCT116 cell lysate using recombinant Cyclin E1 antibody (clone rCCNE1/4936). Predicted molecular weight ~47 kDa.
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Western blot testing of human HCT116 cell lysate using recombinant Cyclin E1 antibody (clone rCCNE1/4936). Predicted molecular weight ~47 kDa.
IHC staining of FFPE human ovarian carcinoma tissue with recombinant Cyclin E antibody (clone rCCNE1/4936). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma tissue with recombinant Cyclin E1 antibody (clone rCCNE1/4936). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Cyclin E1 antibody (clone rCCNE1/4936) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rCCNE1/4936
Purity Protein A/G affinity
UniProt IDP24864
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Cyclin E1 antibody is available for research use only.
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Description

Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Application Notes

Optimal dilution of the recombinant Cyclin E1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 10-176 was used as the immunogen for the Cyclin E1 antibody.

Storage

Aliquot the recombinant Cyclin E1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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