- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant Creatine kinase B Antibody / CK-BB / CKB [clone CKBB/8306R] (V5468)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V5468-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 539 | |
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V5468-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 249 | |
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V5468SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat, Hamster, Guinea pig |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | CKBB/8306R |
| Purity | Protein A/G affinity |
| UniProt | P12277 |
| Localization | Cytoplasm |
| Applications | Western Blot : 2-4ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This recombinant Creatine kinase B antibody is available for research use only. |
|
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Recombinant Creatine kinase B antibody detects Creatine kinase B-type, also known as CK-BB or CKB, a cytosolic enzyme that plays a key role in maintaining cellular energy homeostasis. The UniProt recommended name is Creatine kinase B-type (CKB). This enzyme catalyzes the reversible conversion of ATP and creatine into phosphocreatine and ADP, forming a rapid phosphate-transfer system that replenishes ATP during periods of high energy demand in the brain and other active tissues.
Functionally, Recombinant Creatine kinase B antibody recognizes a 43 kDa homodimeric enzyme that serves as the brain-specific isoform of the creatine kinase family. CKB operates within the phosphocreatine shuttle, transferring high-energy phosphate bonds between mitochondria and cytosolic ATP-dependent processes. This mechanism ensures rapid energy delivery to Na+/K+-ATPase pumps, synaptic vesicles, and cytoskeletal structures that drive neuronal signaling and neurotransmitter release. CKB's efficient ATP buffering capacity allows neurons to maintain electrical activity and synaptic transmission under continuous workload.
The CKB gene is located on chromosome 14q32.33 and encodes a 381-amino-acid enzyme highly conserved across vertebrate species. Structurally, CKB contains ATP- and creatine-binding sites stabilized by magnesium ions, and it functions as a homodimer or heterodimer (CKMB) depending on tissue type. Expression is strongest in brain, retina, and other excitable tissues, while smaller amounts are found in kidney and testis. Under pathological conditions such as stroke, brain trauma, or hypoxia, elevated CKBB levels in serum and cerebrospinal fluid reflect neural cell damage. In cancer research, CKB overexpression has been associated with metabolic adaptation and enhanced tumor cell survival, particularly in small-cell lung carcinoma and colorectal cancer.
Recombinant Creatine kinase B antibody is produced using recombinant technology for high specificity and consistent performance. It is suitable for immunoblotting, immunofluorescence, and ELISA applications exploring metabolic regulation, oxidative stress, and neuroenergetics. CKB interacts with cytoskeletal proteins to channel ATP directly to sites of high energy consumption, coupling mitochondrial output with membrane function. Experimental data show that loss of Ckb impairs synaptic plasticity, decreases phosphocreatine buffering, and heightens sensitivity to energy depletion, confirming its role as a vital regulator of neuronal metabolism.
Outside the nervous system, CKB contributes to energy coupling in sperm motility, smooth muscle contraction, and photoreceptor activity. Its function is modulated by redox state, pH, and post-translational modifications such as phosphorylation. Through targeted detection, Recombinant Creatine kinase B antibody enables studies of energy flux, mitochondrial coordination, and metabolic adaptation in health and disease. NSJ Bioreagents provides this antibody validated for research use in metabolism, neuroscience, and cancer biology.
Optimal dilution of the recombinant Creatine kinase B antibody should be determined by the researcher.
Recombinant human full-length Creatine kinase B protein was used as the immunogen for the recombinant Creatine kinase B antibody.
Aliquot the recombinant Creatine kinase B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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