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Home >> Antibodies >> Recombinant COL4A3 Antibody / Collagen IV

Recombinant COL4A3 Antibody / Collagen IV [clone COL4A3/9395R] (V5400)

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  Catalog No Formulation Size Price (USD)  
Image V5400-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5400-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5400SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
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IHC staining of FFPE human pancreas tissue with Collagen IV antibody (clone COL4/9395R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human pancreas tissue with Collagen IV antibody (clone COL4/9395R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free COL4A antibody (clone COL4/9395R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name COL4A3/9395R
Purity Protein A/G affinity
UniProt P02462 , P08572 , P29400 , P53420
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Collagen IV antibody is available for research use only.
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Description

Collagen Type IV is a major component of the basement membrane and plays an important role in cell adhesion, migration, differentiation and growth. Collagen Type IV express at the basement membranes in a variety of tissues including kidney, muscle, lymph nodes, lung, tendon and spleen. Collagen Type IV has been shown to be useful in differentiating microinvasive from in situ ductal carcinomas of the breast. Other Collagen Type IV studies include use in pancreatic adenocarcinoma and chronic pancreatitis, nephrosclerosis and other kidney diseases, oral squamous cell carcinoma, laryngeal cancers, ovarian cancers and cervical cancers.

Application Notes

Optimal dilution of the Collagen IV antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 1400-1670) of human COL4A protein was used as the immunogen for the Collagen IV antibody.

Storage

Aliquot the Collagen IV antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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