Clusterin Antibody for WB / Secreted Glycoprotein Detection [clone RM437] (R20452)

Clusterin Antibody Brain WB. Western blot analysis of human brain tissue lysate using Clusterin antibody for WB. The recombinant rabbit monoclonal antibody clone RM437 detects a band at approximately 36-39 kDa, consistent with the predicted molecular weight of the processed alpha subunit of Clusterin / CLU. This banding pattern reflects proteolytic cleavage of the heterodimer precursor, while higher molecular weight forms may also be present depending on glycosylation and processing state. Detection in brain tissue aligns with the known expression of this secreted glycoprotein in the central nervous system.

Clusterin Antibody Human Tonsil IHC. Immunohistochemistry analysis of FFPE human tonsil tissue using Clusterin antibody for WB. The recombinant rabbit monoclonal antibody clone RM437 demonstrates strong cytoplasmic and extracellular staining within lymphoid follicles, consistent with Clusterin / CLU expression as a secreted glycoprotein. Signal is enriched in follicular regions and surrounding microenvironment, reflecting accumulation of this processed and secreted protein, while background staining remains low. HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling prior to staining.