- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant CKBB Antibody / Creatine kinase B [clone rCKBB/8844] (V4360)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4360-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 539 | |
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V4360-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 249 | |
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V4360SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat, Hamster, Guinea pig |
| Format | Purified |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rCKBB/8844 |
| Purity | Protein A/G affinity |
| UniProt | P12277 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml |
| Limitations | This recombinant CKBB antibody is available for research use only. |
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Recombinant CKBB antibody detects Creatine kinase B-type, the brain isoform of the creatine kinase family that maintains energy homeostasis by catalyzing the reversible transfer of phosphate between ATP and creatine. The UniProt recommended name is Creatine kinase B-type (CKB), commonly known as CK-BB or brain-type creatine kinase. This enzyme plays a central role in sustaining ATP levels in neurons, glial cells, and other high-energy tissues.
Functionally, Recombinant CKBB antibody identifies a 43 kDa cytosolic enzyme that forms homodimers (CKBB) or heterodimers with the muscle isoform (CKMB). CKB drives the phosphocreatine shuttle, a system that rapidly transfers energy from mitochondria to cellular sites of ATP consumption. Within the nervous system, CKB localizes near Na+/K+-ATPase pumps, synaptic vesicles, and cytoskeletal structures, ensuring efficient ATP regeneration during neural signaling. It supports processes such as neurotransmitter release, vesicle recycling, and ion transport, all of which depend on rapid ATP turnover.
The CKB gene is located on chromosome 14q32.33 and encodes a 381-amino acid protein with conserved catalytic domains responsible for creatine and ATP binding. CKB expression is particularly high in the brain, retina, and smooth muscle, but it is also found in endocrine and reproductive tissues. Dysregulation of CKB has been linked to neurological disorders, brain injury, and tumor metabolism. Increased serum CKBB levels are diagnostic for hypoxic brain injury, trauma, and certain cancers such as small-cell lung carcinoma and neuroblastoma, where elevated CKB supports proliferation and resistance to metabolic stress.
Recombinant CKBB antibody is a high-affinity reagent produced using recombinant technology to ensure consistency and lot-to-lot reliability. It is validated for western blotting, immunofluorescence, and immunohistochemistry applications across human, mouse, and rat samples. The antibody is ideal for studies of neuronal metabolism, mitochondrial coupling, and oxidative stress. CKB interacts with cytoskeletal proteins and membrane components to localize energy production precisely where ATP demand is highest. Experimental models show that Ckb knockout results in cognitive impairment, reduced energy buffering, and increased sensitivity to ischemia, underscoring its physiological importance.
Beyond neural tissue, CKB contributes to sperm motility, retinal signaling, and smooth muscle contraction, emphasizing its broad role in maintaining localized energy flux. Through accurate detection of this enzyme, Recombinant CKBB antibody provides valuable insight into cellular energy dynamics, neuroprotection, and tumor metabolism. NSJ Bioreagents offers this recombinant antibody fully validated for metabolic, neurological, and oncological research applications.
Optimal dilution of the recombinant CKBB antibody should be determined by the researcher.
Recombinant human full-length protein was used as the immunogen for the recombinant CKBB antibody.
Aliquot the recombinant CKBB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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