- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CKBB Antibody / Creatine kinase B [clone rCKBB/8842] (V4374)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4374-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4374-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4374SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat, Hamster, Guinea pig |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rCKBB/8842 |
| Purity | Protein A/G affinity |
| UniProt | P12277 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml |
| Limitations | This CKBB antibody is available for research use only. |
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CKBB antibody detects Creatine kinase B-type, also known as brain-type creatine kinase or CKB, an ATP-buffering enzyme that maintains cellular energy stability in tissues with rapid and fluctuating energy needs. The UniProt recommended name is Creatine kinase B-type (CKB). This cytosolic phosphotransferase catalyzes the reversible reaction of ATP and creatine to form phosphocreatine and ADP, providing an immediate source of high-energy phosphate to sustain essential cellular processes.
Functionally, CKBB antibody recognizes a 43 kDa enzyme that forms homodimers (CKBB) and cooperates with mitochondrial creatine kinases to transfer phosphate groups between energy-producing and energy-consuming sites. Within neurons, CKB is positioned near Na+/K+-ATPase pumps, vesicle membranes, and cytoskeletal assemblies, ensuring continuous ATP regeneration for neurotransmission and membrane potential maintenance. Its role in the phosphocreatine shuttle enables fast recycling of energy during periods of intense synaptic activity and protects cells from ATP depletion under metabolic stress.
The CKB gene is located on chromosome 14q32.33 and encodes a 381-amino acid enzyme highly conserved across mammals. CKB expression is most prominent in brain, retina, and other excitable tissues, though detectable levels occur in smooth muscle, endocrine cells, and reproductive organs. Elevated CKBB concentrations in serum or cerebrospinal fluid indicate neuronal injury, ischemia, or neurodegeneration. Overexpression of CKB has also been linked to tumor progression in small-cell lung carcinoma, colorectal cancer, and glioblastoma, where it supports ATP turnover and enhances cell motility.
CKBB antibody is produced using recombinant expression technology to ensure high purity, reproducibility, and reduced cross-reactivity compared with conventional polyclonal antibodies. It is validated for western blotting, immunofluorescence, immunohistochemistry, and ELISA applications. This reagent is ideal for analyzing brain metabolism, oxidative stress response, and bioenergetic adaptation. Studies using Ckb knockout models demonstrate severe deficits in cognitive performance, impaired energy buffering, and increased sensitivity to hypoxia, confirming the enzyme's essential function in neural physiology.
Outside the nervous system, CKB supports energy coupling in sperm flagella, retinal photoreceptors, and smooth muscle cells. Regulation occurs through phosphorylation, oxidation, and transcriptional control by CREB, NRF1, and Sp1. By providing reliable detection of this energy-regulating enzyme, the recombinant CKBB antibody is a powerful tool for investigating metabolic resilience, mitochondrial communication, and cancer cell energetics. NSJ Bioreagents supplies this recombinant antibody fully validated for use in human, mouse, and rat samples across metabolic and neurological research fields.
This Creatine Kinase B Antibody is part of a broader Creatine Kinase B antibody panel offered by NSJ Bioreagents.
Optimal dilution of the CKBB antibody should be determined by the researcher.
Recombinant human full-length protein was used as the immunogen for the recombinant CKBB antibody.
Aliquot the CKBB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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