CK5 Antibody RM226 / Recombinant Cytokeratin 5 Antibody [clone RM226] (R20256)

Description

CK5 Antibody RM226 recognizes cytokeratin 5 (KRT5), a type II intermediate filament protein expressed primarily in basal epithelial cells of stratified and pseudostratified epithelia. Also known as keratin 5, CK5 is a structural component of the cytoskeleton that helps epithelial cells withstand mechanical stress. The human KRT5 gene is located on chromosome 12 and encodes a protein with a molecular weight of approximately 58 kDa. KRT5 expression is particularly prominent in basal keratinocytes of the epidermis, as well as basal epithelial populations in the prostate, mammary gland, and respiratory tract.

KRT5 typically pairs with keratin 14 (KRT14), a type I intermediate filament protein, to form heterodimers that assemble into larger filament networks. These networks contribute to cellular stability and help maintain epithelial tissue architecture. As basal keratinocytes differentiate and migrate toward the skin surface, expression of KRT5 and KRT14 generally decreases while suprabasal keratins such as KRT1 and KRT10 become more prominent. This differentiation-dependent expression pattern makes CK5 useful for identifying basal epithelial compartments and investigating epithelial development, regeneration, and wound healing.

In cancer research, CK5 is widely studied as a marker of squamous differentiation and basal-like epithelial phenotypes. Its expression is frequently detected in squamous cell carcinomas of the lung, cervix, head, and neck. CK5 is commonly evaluated alongside CK6 and p63 when investigating squamous differentiation. In breast cancer research, CK5 expression can help characterize basal-like tumor populations, although additional molecular and immunohistochemical markers are necessary for accurate classification. In prostate tissue, CK5 is associated with basal epithelial cells and can help distinguish preserved basal cell layers from malignant glands, which typically lack these cells.

Mutations in KRT5 are associated with epidermolysis bullosa simplex, an inherited disorder characterized by skin fragility and blister formation. NSJ Bioreagents offers recombinant rabbit monoclonal CK5 Antibody RM226, developed against a peptide corresponding to the C-terminal region of human KRT5. Recombinant monoclonal production provides a defined antibody sequence and supports manufacturing consistency. A CK5 Antibody RM226 is useful for investigating basal epithelial differentiation, cytoskeletal organization, and KRT5 expression in normal and diseased tissues.

Explore our protein microarray-validated Cytokeratin 5 Antibody KRT5/3594 for investigating basal epithelial differentiation, squamous cell carcinomas, and keratin filament organization.

Application Notes

The stated application concentrations are suggested starting points. Titration of the CK5 Antibody RM226 / Recombinant Cytokeratin 5 Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the C-terminus of human Cytokeratin 5 was used as the immunogen for this recombinant CK5 Antibody RM226.

Storage

Store the CK5 Antibody RM226 at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Alternate Names

Cytokeratin 5 antibody, Keratin 5 antibody, KRT5 antibody, CK-5 antibody, Basal Cell Keratin antibody, Type II Cytoskeletal Keratin 5 antibody