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Home >> Antibodies >> Recombinant CK19 Antibody / Cytokeratin 19

Recombinant CK19 Antibody / Cytokeratin 19 [clone rKRT19/800] (V3613)

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  Catalog No Formulation Size Price (USD)  
Image V3613-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3613-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3613SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3613IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recmousemono
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Western blot testing of human HepG2 cell lysate using recombinant CK19 antibody (clone rKRT19/800). Predicted molecular weight ~43 kDa.
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Western blot testing of human HepG2 cell lysate using recombinant CK19 antibody (clone rKRT19/800). Predicted molecular weight ~43 kDa.
Western blot testing of human lung lysate using recombinant CK19 antibody (clone rKRT19/800). Predicted molecular weight ~43 kDa.
IHC testing of FFPE human colon carcinoma with recombinant CK19 antibody (clone rKRT19/800). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant CK19 antibody (clone rKRT19/800) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rKRT19/800
Purity Protein G affinity chromatography
UniProt P08727
Localization Cytoplasmic, cell membrane, secreted
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant CK19 antibody is available for research use only.
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Description

Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

Application Notes

Optimal dilution of the recombinant CK19 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human Cytokeratin 19 protein was used as the immunogen for the recombinant CK19 antibody.

Storage

Store the recombinant CK19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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