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Home >> Antibodies >> Recombinant CK19 Antibody / Cytokeratin 19

Recombinant CK19 Antibody / Cytokeratin 19 [clone KRT19/8091R] (V4485)

  Catalog No Formulation Size Price (USD)  
Image V4485-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4485-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4485SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human prostate cancer tissue with recombinant CK19 antibody (clone KRT19/8091R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CK19 antibody (clone KRT19/8091R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse and Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name KRT19/8091R
Purity Protein A/G affinity
UniProt P08727
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant CK19 antibody is available for research use only.
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Description

Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

Application Notes

Optimal dilution of the recombinant CK19 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human protein was used as the immunogen for the recombinant CK19 antibody.

Storage

Aliquot the recombinant CK19 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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