CK10 Antibody KRT10/1948R / Recombinant Cytokeratin 10 Antibody [clone KRT10/1948R] (V3632)

CK10 Antibody KRT10/1948R by IHC in Human Skin. Immunohistochemistry of FFPE human skin tissue using CK10 Antibody KRT10/1948R. Strong brown cytoplasmic staining is observed in the suprabasal keratinocytes of the epidermis, particularly within the spinous and granular layers, while the basal cell layer is comparatively negative. The Recombinant Cytokeratin 10 Antibody highlights the distribution of differentiated keratinocytes in stratified squamous epithelium. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

CK10 Antibody KRT10/1948R by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free CK10 Antibody KRT10/1948R under non-reducing (NR) and reducing (R) conditions, with 2.5 ug of antibody loaded per lane. The non-reducing lane shows a prominent band migrating at approximately 150 kDa, consistent with intact IgG. Under reducing conditions, bands are observed at approximately 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains, respectively. The Recombinant Cytokeratin 10 Antibody demonstrates the expected electrophoretic profile of a purified rabbit monoclonal antibody preparation.

CK10 Antibody KRT10/1948R by WB in Skin Tissue Lysates from Multiple Species. Western blot analysis of skin tissue lysates using CK10 Antibody KRT10/1948R. Six lanes contain human, mouse, rat, hamster, guinea pig, and rabbit skin lysates, respectively. A prominent immunoreactive band is detected at approximately 56-60 kDa in lanes 1-3, corresponding to human, mouse, and rat samples. No distinct band is observed in lanes 4-6, containing hamster, guinea pig, and rabbit samples. The observed molecular weight is consistent with the expected size of KRT10. The Recombinant Cytokeratin 10 Antibody demonstrates detection of endogenous keratin 10 in human and rodent skin tissues under the tested conditions.

CK10 Antibody KRT10/1948R by WB in Human Skin. Western blot analysis of human skin tissue lysate using CK10 Antibody KRT10/1948R. A prominent immunoreactive band is detected at approximately 56-60 kDa, consistent with the expected molecular weight of cytokeratin 10. The Recombinant Cytokeratin 10 Antibody demonstrates detection of endogenous KRT10 in human skin, where this intermediate filament protein is predominantly expressed in differentiated suprabasal keratinocytes. The relatively clean banding pattern supports recognition of the expected target protein under the tested conditions.