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Home >> Antibodies >> Recombinant Chromogranin A Antibody

Recombinant Chromogranin A Antibody [clone bGRAN2R] (V3720)

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  Catalog No Formulation Size Price (USD)  
Image V3720-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3720-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3720SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3720IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC analysis of FFPE human parathyroid gland stained with recombinant Chromogranin A antibody (clone bGRAN2R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min and allow to cool at RT for 20 min before testing.
IHC analysis of FFPE human parathyroid gland stained with recombinant Chromogranin A antibody (clone bGRAN2R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min and allow to cool at RT for 20 min before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name bGRAN2R
Purity Protein A affinity chromatography
UniProt P10645
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant Chromogranin A antibody is available for research use only.
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Description

Chromogranin A or parathyroid secretory protein 1 (gene name CHGA) is a member of the granin family of neuroendocrine secretory proteins, i.e., it is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A is the precursor to several functional peptides including vasostatin-1, vasostatin-2, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). [Wiki]

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Chromogranin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant human protein was used as the immunogen for the recombinant Chromogranin A antibody.

Storage

Store the recombinant Chromogranin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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