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Home >> Antibodies >> Recombinant CFTR Antibody / Cystic Fibrosis Transmembrane Regulator

Recombinant CFTR Antibody / Cystic Fibrosis Transmembrane Regulator [clone CFTR/7003R] (V9382)

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  Catalog No Formulation Size Price (USD)  
Image V9382-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9382-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9382SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human kidney tissue with recombinant CFTR antibody (clone CFTR/7003R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human kidney tissue with recombinant CFTR antibody (clone CFTR/7003R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic tissue with recombinant CFTR antibody (clone CFTR/7003R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian carcinoma tissue with recombinant CFTR antibody (clone CFTR/7003R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CFTR antibody (clone CFTR/7003R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CFTR/7003R
Purity Protein A/G affinity
UniProt P13569
Localization Cell surface, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CFTR antibody is available for research use only.
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Description

Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Application Notes

Optimal dilution of the recombinant CFTR antibody should be determined by the researcher.

Immunogen

A portion of amino acids 550-850 was used as the immunogen for the recombinant CFTR antibody.

Storage

Aliquot the recombinant CFTR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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