CDX2 Antibody [clone CDX2/4394R] (V8630)

IHC analysis of CDX2 / Caudal type homeobox 2 antibody in human colon carcinoma tissue. Formalin-fixed, paraffin-embedded human colon carcinoma shows strong nuclear HRP-DAB brown chromogenic staining in malignant epithelial cells, consistent with intestinal differentiation, while surrounding stromal and inflammatory cells are largely negative. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling prior to staining.

SDS-PAGE analysis of purified, BSA-free CDX2 antibody as confirmation of integrity and purity.

IHC analysis of CDX2 / Caudal type homeobox 2 antibody in human colon adenocarcinoma tissue. Formalin-fixed, paraffin-embedded human colon adenocarcinoma shows strong nuclear HRP-DAB brown chromogenic staining in tumor epithelial cells, consistent with intestinal lineage expression of CDX2, while surrounding stromal elements are negative. Clone CDX2/4394R was used at 2 ug/ml in PBS for 30 min at RT. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95oC followed by cooling at RT for 20 minutes.

Negative control: IHC analysis of CDX2 / Caudal type homeobox 2 antibody in human tonsil tissue. Formalin-fixed, paraffin-embedded human tonsil shows absence of nuclear HRP-DAB brown chromogenic staining in lymphoid cells, consistent with lack of CDX2 expression in non-intestinal tissue. This section serves as a negative tissue control using clone CDX2/4394R at 2 ug/ml in PBS for 30 min at RT. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95oC followed by cooling at RT for 20 minutes.