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Home >> Antibodies >> Recombinant CDH17 Antibody / Cadherin 17

Recombinant CDH17 Antibody / Cadherin 17 [clone rCDH17/9677] (V5591)

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  Catalog No Formulation Size Price (USD)  
Image V5591-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5591-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5591SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recmousemono
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Western blot testing of human small intestine tissue lysate with recombinant CDH17 antibody. Predicted molecular weight ~92 kDa but may be observed at higher molecular weights due to glycosylation.
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Western blot testing of human small intestine tissue lysate with recombinant CDH17 antibody. Predicted molecular weight ~92 kDa but may be observed at higher molecular weights due to glycosylation.
IHC staining of FFPE human colon tissue with recombinant CDH17 antibody (clone rCDH17/9677). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CDH17 antibody (clone rCDH17/9677) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rCDH17/9677
Purity Protein A/G affinity
UniProt Q12864
Localization Cytoplasm, Cell membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This recombinant CDH17 antibody is available for research use only.
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Description

It recognizes a protein of 120kDa, which is identified as Cadherin 17 (also known as LI Cadherin). The cadherins are a family of Calcium-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. LI-cadherin (for liver-intestine-cadherin) expression is restricted to liver and intestine tissues and is specifically localized to the basolateral domain of hepatocytes and enterocytes.

Application Notes

Optimal dilution of the recombinant CDH17 antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 242-418) of human Cadherin 17 protein was used as the immunogen for the recombinant CDH17 antibody.

Storage

Aliquot the recombinant CDH17 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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