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Home >> Antibodies >> Recombinant Cdc20 Antibody

Recombinant Cdc20 Antibody [clone CDC20/7026R] (V9446)

  Catalog No Formulation Size Price (USD)  
Image V9446-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9446-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9446SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human kidney tissue with recombinant Cdc20 antibody CDC20/7026R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney carcinoma tissue with recombinant Cdc20 antibody (clone CDC20/7026R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant Cdc20 antibody (clone CDC20/7026R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Cdc20 antibody (clone CDC20/7026R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CDC20/7026R
Purity Protein A/G affinity
UniProt Q12834
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Cdc20 antibody is available for research use only.
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Description

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. [UniProt]

Application Notes

Optimal dilution of the recombinant Cdc20 antibody should be determined by the researcher.

Immunogen

Recombinant human Cdc20 protein was used as the immunogen for the recombinant Cdc20 antibody.

Storage

Aliquot the recombinant Cdc20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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