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Home >> Antibodies >> Recombinant CD8A Antibody

Recombinant CD8A Antibody [clone CD8/4391R] (V8519)

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  Catalog No Formulation Size Price (USD)  
Image V8519-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 539
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V8519-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 249
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V8519SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human lymph node tissue with recombinant CD8A antibody (clone CD8/4391R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lymph node tissue with recombinant CD8A antibody (clone CD8/4391R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with recombinant CD8A antibody (clone CD8/4391R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD8A antibody (clone CD8/4391R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD8A antibody (clone CD8/4391R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant CD8A antibody (clone CD8/4391R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant CD8A antibody (clone CD8/4391R). These results demonstrate the foremost specificity of the CD8/4391R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name CD8/4391R
Purity Protein A affinity chromatography
UniProt P01732
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant CD8A antibody is available for research use only.
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Description

Recombinant CD8A antibody detects CD8 alpha, a surface glycoprotein encoded by the CD8A gene. CD8 is expressed on cytotoxic T lymphocytes and a subset of natural killer cells, where it acts as a coreceptor for MHC class I molecules. By stabilizing interactions between T-cell receptors and antigen-MHC complexes, CD8 lowers the threshold for T-cell activation. Because of its central role in cytotoxic immunity, Recombinant CD8A antibody is widely used in immunology, oncology, and infectious disease research.

CD8 alpha forms either homodimers or heterodimers with CD8 beta. Its extracellular immunoglobulin-like domain binds to non-polymorphic regions of MHC class I, while its cytoplasmic tail associates with kinases such as Lck. This arrangement ensures efficient antigen recognition and rapid activation of cytotoxic T cells. CD8 expression defines a major T-cell lineage that is indispensable for immune defense against viral infections and malignant cells.

The Recombinant CD8A antibody clone CD8/4391R provides specific and reproducible detection. Recombinant technology guarantees lot-to-lot consistency, reducing variability across experiments. Clone CD8/4391R has been cited in peer-reviewed studies of T-cell biology, immune surveillance, and tumor immunology. Its versatility supports applications in flow cytometry, immunohistochemistry, and cytotoxicity assays.

Research using clone CD8/4391R has highlighted how CD8+ T cells mediate direct cytotoxic responses through perforin and granzymes, while also producing cytokines that shape broader immune reactions. In oncology, detection of CD8 infiltration into tumor tissue is a strong predictor of prognosis and therapeutic response. In infectious disease, CD8 analysis has provided insight into antiviral immunity and vaccine efficacy. Its reproducibility and specificity make this antibody an essential reagent for immune monitoring.

NSJ Bioreagents provides this Recombinant CD8A antibody to support immunology, oncology, and infectious disease research. Alternate names include CD8 alpha chain antibody, T-cell surface glycoprotein CD8 alpha antibody, MHC class I coreceptor antibody, cytotoxic T-cell marker antibody, and CD8A molecule antibody.

Application Notes

Optimal dilution of the recombinant CD8A antibody should be determined by the researcher.

Immunogen

A portion of amino acids 135-235 from the human protein was used as the immunogen for the recombinant CD8A antibody.

Storage

Store the recombinant CD8A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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