CD8A Antibody / Human Protein Microarray Specificity Validated Antibody [clone CD8/4391R] (V8519)

CD8A Antibody Lymph Node IHC. Immunohistochemistry analysis of CD8 alpha (CD8A) in FFPE human lymph node tissue shows strong membranous staining of abundant cytotoxic T lymphocytes within lymphoid regions, consistent with expected localization of CD8-positive T cells in secondary lymphoid tissue and supporting identification of immune cell populations in a densely cellular microenvironment. Human protein microarray specificity validated antibody performance supports high-confidence, target-selective detection in immune-rich tissues (clone CD8/4391R). Heat-induced epitope retrieval was performed in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human lymph node tissue with recombinant CD8A antibody (clone CD8/4391R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant CD8A antibody (clone CD8/4391R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

CD8A Antibody Tonsil IHC. Immunohistochemistry analysis of CD8 alpha (CD8A) in FFPE human tonsil tissue shows strong membranous staining of cytotoxic T lymphocytes distributed within lymphoid regions, consistent with expected localization of CD8-positive T cells and supporting identification of immune cell populations in secondary lymphoid tissue. Human protein microarray specificity validated antibody performance supports high-confidence, target-selective detection in immune-rich samples (clone CD8/4391R). Heat-induced epitope retrieval was performed in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

Negative control: IHC staining of FFPE human brain tissue with recombinant CD8A antibody (clone CD8/4391R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

CD8A Antibody Specificity Validation. Human protein microarray specificity analysis of CD8 alpha (CD8A) using a panel of more than 19,000 full-length human proteins demonstrates a dominant top-ranked signal for CD8A with strong separation from lower-ranking proteins, supporting high specificity and minimal off-target binding of the CD8/4391R monoclonal antibody. The signal distribution confirms selective target recognition consistent with specificity-focused validation (clone CD8/4391R). Z-score represents signal strength in standard deviations above the array mean, while S-score reflects the difference between ranked signals and indicates relative target specificity.