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Home >> Antibodies >> Recombinant CD86 Antibody

Recombinant CD86 Antibody [clone rC86/6872] (V9521)

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  Catalog No Formulation Size Price (USD)  
Image V9521-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9521-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9521SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human lymph node with recombinant CD86 antibody (clone rC86/6872) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lymph node with recombinant CD86 antibody (clone rC86/6872) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD86 antibody (clone rC86/6872) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rC86/6872
Purity Protein A/G affinity
UniProt P4208
Localization Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CD86 antibody is available for research use only.
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Description

Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkins disease.

Application Notes

Optimal dilution of the recombinant CD86 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 66-195 was used as the immunogen for the recombinant CD86 antibody.

Storage

Aliquot the recombinant CD86 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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