• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> Recombinant CD86 Antibody / Rabbit Monoclonal

Recombinant CD86 Antibody / Rabbit Monoclonal [clone CDLA86-2R] (V3673)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image V3673-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
Image
V3673-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
Image
V3673SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Image
V3673IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
Bulk quote request
IHC testing of FFPE human colon with recombinant CD86 antibody (CDLA86-2R). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
›
IHC testing of FFPE human colon with recombinant CD86 antibody (CDLA86-2R). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human Ramos cells with recombinant CD86 antibody (blue, clone CDLA86-2R) and isotype control (red).
IF/ICC staining of human Raji cells with recombinant CD86 antibody (green, clone CDLA86-2R) and Reddot nuclear stain (red).
Western blot testing of human Ramos cell lysate with recombinant CD86 antibody (clone CDLA86-2R). Expected molecular weight: 38-70 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human. Other species not tested.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CDLA86-2R
Purity Protein A affinity chromatography
UniProt P42081
Localization Cytoplasmic, membrane
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence/Immunocytochemistry : 2-4ug/ml
Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant CD86 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : FACS, IF, IHC-P
    Reactivity : Human, Mouse, Rat
    Recmousemono
  • Applications : ELISA, FACS, IF, WB, IHC-P
    Reactivity : Human, Mouse, Rat
    Recrabbitmono
  • Applications : IHC-P
    Reactivity : Human
    Recrabbitmono
  • Applications : IHC-P
    Reactivity : Human
    Recmousemono
  • Applications : WB, IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : WB, IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : FACS
    Reactivity : Human
    Citation  Citations(10)
  • Applications : Functional studies, FACS, IF, IHC-P
    Reactivity : Human, Mouse, Rat
    Citation  Citations(11)
  • Applications : FACS, IF
    Reactivity : Human, Mouse and Rat. Other species not known.
    Citation  Citations(11)
  • Applications : IF, WB, IHC-P
    Reactivity : Human
  • Applications : IF, IHC-P
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P
    Reactivity : Human, Mouse
  • Applications : WB, ELISA
    Reactivity : Human
  • Applications : WB, ELISA
    Reactivity : Mouse, Rat

Description

Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Application Notes

Optimal dilution of the recombinant CD86 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human full-length protein was used as the immunogen for the recombinant CD86 antibody.

Storage

Store the recombinant CD86 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all CD86 antibodies

   2.   View all recombinant antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.