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Home >> Antibodies >> Recombinant CD86 Antibody

Recombinant CD86 Antibody [clone C86/6500R] (V9474)

  Catalog No Formulation Size Price (USD)  
Image V9474-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9474-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9474SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human lymph node tissue with recombinant CD86 antibody (clone C86/6500R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with recombinant CD86 antibody (clone C86/6500R) at 2ug/ml in PBS for 30min RT. Staining of cell surface is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD86 antibody (clone C86/6500R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name C86/6500R
Purity Protein A/G affinity
UniProt P4208
Localization Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CD86 antibody is available for research use only.
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Description

CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes.

Application Notes

Optimal dilution of the recombinant CD86 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 66-195 was used as the immunogen for the recombinant CD86 antibody.

Storage

Aliquot the recombinant CD86 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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