CD79a Antibody Rabbit Monoclonal [clone IGA/1688R] (V3482)

Description

CD79a antibody recognizes CD79a, a type I transmembrane protein encoded by the CD79A gene and also known as Ig-alpha and MB-1. CD79a is a critical component of the B cell receptor complex, forming a heterodimer with CD79b to associate with membrane-bound immunoglobulin. CD79a antibody is widely used in research focused on B lymphocyte development, differentiation, and B cell-derived malignancies. The protein is localized primarily to the cell membrane in mature B cells and can also be detected in the cytoplasm of precursor B cells during early stages of differentiation.

CD79a contains an extracellular immunoglobulin-like domain, a single transmembrane region, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based activation motif. Upon antigen binding to surface immunoglobulin, the CD79a/CD79b complex transduces intracellular signaling through phosphorylation of this activation motif, leading to recruitment of downstream kinases and activation of pathways involved in B cell proliferation and survival. CD79a expression begins early in B cell ontogeny, preceding surface immunoglobulin expression, and is maintained throughout most stages of B cell maturation but is typically absent in plasma cells.

Because of its lineage-restricted expression, CD79a is an important marker in hematopathology research. It is commonly detected in normal B lymphocytes within lymphoid tissues and in B cell-derived lymphomas and leukemias. In tissue sections, immunostaining typically demonstrates membranous and cytoplasmic localization in B cells, while non-lymphoid tissues are negative. Clone IGA/1688R is a recombinant rabbit monoclonal antibody produced through defined sequence expression, supporting lot-to-lot consistency. This CD79a antibody enables investigation of B cell receptor biology, B cell lineage identification, and studies of lymphoid neoplasms in research applications.

Application Notes

Optimal dilution of the CD79a antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for the CD79a antibody.

Storage

Store the CD79a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).