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Home >> Antibodies >> Recombinant CD74 Antibody

Recombinant CD74 Antibody [clone rCLIP/813] (V8374)

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  Catalog No Formulation Size Price (USD)  
Image V8374-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8374-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8374SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil with recombinant CD74 antibody (clone rCLIP/813). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with recombinant CD74 antibody (clone rCLIP/813). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD74 antibody (clone rCLIP/813) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rCLIP/813
Purity Protein G affinity chromatography
UniProt P044233
Localization Cell surface and paranuclear globular
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant CD74 antibody is available for research use only.
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Description

It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Application Notes

Optimal dilution of the recombinant CD74 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human CD74 protein was used as the immunogen for the recombinant CD74 antibody.

Storage

Store the recombinant CD74 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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