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Home >> Antibodies >> Recombinant CD68 Antibody

Recombinant CD68 Antibody [clone CDLA68-3R] (V3996)

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  Catalog No Formulation Size Price (USD)  
Image V3996-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3996-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3996SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3996IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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Western blot testing of human spleen lysate with CD68 antibody (clone CDLA68-3R). Expected molecular weight: 37-110 kDa depending on glycosylation level.
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Western blot testing of human spleen lysate with CD68 antibody (clone CDLA68-3R). Expected molecular weight: 37-110 kDa depending on glycosylation level.
IHC staining of FFPE human tonsil with recombinant CD68 antibody (clone CDLA68-3R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CDLA68-3R
Purity Protein A affinity chromatography
UniProt P34810
Localization Cytoplasmic, membrane
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant CD68 antibody is available for research use only.
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Description

CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant CD68 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length recombinant human protein was used as the immunogen for the recombinant CD68 antibody.

Storage

Store the recombinant CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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