Recombinant CD63 Antibody / LAMP-3 [clone rMX-49.129.5] (V7388)

Description

Recombinant CD63 antibody detects CD63, a member of the tetraspanin family encoded by the CD63 gene. CD63 is a glycoprotein characterized by four transmembrane domains and is enriched in late endosomes, lysosomes, and exosomes. It plays key roles in vesicular trafficking, membrane organization, and intercellular communication. Because CD63 serves as a well-established marker for exosomes and extracellular vesicles, Recombinant CD63 antibody is essential in cell biology, immunology, and cancer research.

CD63 contributes to the regulation of integrin signaling, adhesion, and immune cell activation. By organizing tetraspanin-enriched microdomains, it helps cluster receptors and signaling molecules on the plasma membrane. In intracellular compartments, CD63 regulates vesicle fusion and trafficking, ensuring proper sorting of cargo to lysosomes and exosomes. This dual localization underlies its importance in both intracellular and extracellular communication.

The Recombinant CD63 antibody clone rMX-49.129.5 provides consistent and specific recognition. Recombinant production ensures uniformity across batches, minimizing variability in experiments. Clone rMX-49.129.5 has been cited in peer-reviewed studies addressing exosome biology, cancer metastasis, and immune modulation. Its reproducibility supports applications including flow cytometry, immunohistochemistry, and Western blotting.

Research using clone rMX-49.129.5 has shown how CD63 serves as a canonical exosome marker, allowing investigators to quantify extracellular vesicles and track their role in intercellular signaling. In oncology, CD63 detection has clarified how tumor-derived exosomes influence metastasis, angiogenesis, and immune evasion. In immunology, CD63 regulates degranulation in basophils, mast cells, and platelets, linking it to allergic responses and hemostasis. These studies highlight the broad scope of CD63 research.

NSJ Bioreagents supplies this Recombinant CD63 antibody to support exosome biology, immunology, and oncology. Alternate terms include tetraspanin 30 antibody, melanoma-associated antigen ME491 antibody, lysosomal-associated membrane protein antibody, extracellular vesicle marker antibody, and late endosomal tetraspanin antibody.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD63 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length human CD63 was used as the immunogen for this recombinant CD63 antibody.

Storage

Store the recombinant CD63 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).