CD56 Antibody Recombinant Rabbit MAb NCAM1/8392R / NCAM1 Antibody [clone NCAM1/8392R] (V4193)

CD56 Antibody Recombinant Rabbit MAb NCAM1/8392R. Immunohistochemistry of FFPE human colon tissue demonstrates membranous and cytoplasmic staining in scattered neural and neuroendocrine-associated cell clusters consistent with expression of Neural cell adhesion molecule 1 (NCAM1), also known as CD56. Brown chromogenic signal highlights CD56-positive cells within localized structures embedded in the surrounding stromal tissue, while most epithelial and stromal cells show minimal staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

SDS-PAGE analysis of purified, BSA-free recombinant CD56 antibody (clone NCAM1/8392R) as confirmation of integrity and purity.

CD56 Antibody Recombinant Rabbit MAb NCAM1/8392R. Western blot analysis of human HCT-116 cell lysate demonstrates a strong band cluster between approximately 120-180 kDa corresponding to Neural cell adhesion molecule 1 (NCAM1), also known as CD56. These bands are consistent with the major transmembrane NCAM isoforms commonly detected in mammalian cells. Additional signal near approximately 110 kDa may represent a soluble or processed NCAM fragment. The band pattern reflects the presence of multiple NCAM1 isoforms and the heavily glycosylated nature of this cell adhesion molecule.

Western blot testing of human brain tissue lysate with recombinant CD56 antibody. Predicted molecular weight: ~110 kDa (soluble fragment), ~120/125 kDa (GPI-anchored), 140/180 kDa (transmembrane isoforms).