CD5 Antibody / Tumor Immune Microenvironment Marker Antibody [clone C5/4561R] (V9516)

CD5 Antibody for IHC. Immunohistochemistry analysis of CD5 antibody staining in FFPE human tonsil tissue using a tumor immune microenvironment marker antibody, clone C5/4516R. Strong membranous staining is observed in interfollicular T lymphocytes with dense labeling of T cell zones surrounding germinal centers, while follicular B cell areas remain largely negative. The staining highlights immune cell distribution within lymphoid compartments and reflects the role of CD5 in identifying T cell populations relevant to immune infiltration. Negative control inset shows PBS used in place of primary antibody with no specific staining, confirming secondary antibody specificity. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.

CD5 Antibody for IHC. Immunohistochemistry analysis of CD5 antibody staining in FFPE human lymph node tissue using a tumor immune microenvironment marker antibody, clone C5/4561R. Strong membranous staining is observed in T lymphocytes within paracortical regions, with dense labeling of T cell-rich zones surrounding follicles, while germinal center B cell areas remain largely negative. The staining pattern highlights lymph node architecture and reflects the role of CD5 in identifying T cell populations relevant to immune infiltration and tissue-based immune context. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.

Negative control: IHC staining of FFPE human brain with recombinant CD5 antibody (clone C5/4561R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.