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Home >> Antibodies >> Recombinant CD5 Antibody

Recombinant CD5 Antibody [clone C5/4561R] (V9516)

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  Catalog No Formulation Size Price (USD)  
Image V9516-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9516-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9516SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with recombinant CD5 antibody (clone C5/4516R) at 2ug/ml in PBS for 30min RT. Strong surface staining observed. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with recombinant CD5 antibody (clone C5/4516R) at 2ug/ml in PBS for 30min RT. Strong surface staining observed. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with recombinant CD5 antibody (clone C5/4561R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain with recombinant CD5 antibody (clone C5/4561R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name C5/4561R
Purity Protein A/G affinity
UniProt P06127
Localization Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CD5 antibody is available for research use only.
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Description

The CD5 antigen is a transmembrane glycoprotein expressed on the surface of practically all mature human T-cells (about 10% of CD4+ T-cells being CD5 negative). In immature (CD34+) T-cells, CD5 is weakly expressed, the intensity of expression increasing with maturation. CD5 is also expressed in a small subset of normal human B-cells (20% of B-cells in the peripheral blood, scattered cells in the lymph node mantle zone). The CD5+ cells are probably involved in B-T interaction and their ligand is CD72 which is expressed on all B cells. It appears that CD5+ B-cells on activation primarily produce IgM. They also produce more autoantibodies than normal CD5 negative B-cells. Thus, the CD5+ B-cell population is expanded in rheumatoid arthritis and systemic lupus erythematosus. The CD5 antibody reacts with CD5 expressed on B and T cells, and may be a useful aid for the classification of B and T-cell malignancies. This includes B-cell chronic lymphoid leukemia (B-CLL), B-cell small lymphocytic lymphoma (B-SLL), mantle cell lymphoma (MCL) and T-cell lymphoma and leukemia.

Application Notes

Optimal dilution of the recombinant CD5 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 450-495 from the intracellular region of human CD5 was used as the immunogen for the recombinant CD5 antibody.

Storage

Aliquot the recombinant CD5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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