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Home >> Antibodies >> Recombinant CD45RB Antibody

Recombinant CD45RB Antibody [clone rPTPRC/1132] (V8045)

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  Catalog No Formulation Size Price (USD)  
Image V8045-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8045-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8045SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human lymph node with recombinant CD45RB antibody (clone rPTPRC/1132). HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.
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IHC staining of FFPE human lymph node with recombinant CD45RB antibody (clone rPTPRC/1132). HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human spleen lysate with recombinant CD45RB antibody (clone rPTPRC/1132). Expected molecular weight: 147-220 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free recombinant CD45RB antibody (clone rPTPRC/1132) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rPTPRC/1132
Purity Protein G affinity chromatography
UniProt P08575
Localization Cell surface, cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CD45RB antibody is available for research use only.
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Description

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.

Application Notes

Optimal dilution of the recombinant CD45RB antibody should be determined by the researcher.

Immunogen

Recombinant full-length human CD45RB protein was used as the immunogen for the recombinant CD45RB antibody.

Storage

Store the recombinant CD45RB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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