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Home >> Antibodies >> Recombinant CD45 Antibody / Rabbit Monoclonal

Recombinant CD45 Antibody / Rabbit Monoclonal [clone PTPRC/1975R] (V3656)

  Catalog No Formulation Size Price (USD)  
Image V3656-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V3656-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3656SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V3656IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of recombinant CD45 antibody and FFPE human lymph node (clone PTPRC/1975R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of PFA-fixed human Raji cells with CD45 antibody (clone PTPRC/1975R); Red=isotype control, Blue= CD45 antibody.
Western blot testing of human spleen lysate with CD45 antibody (clone PTPRC/1975R). Expected molecular weight: 147-220 kDa depending on glycosylation level.
Immunofluorescence staining of human Raji cells with CD43 antibody (clone PTPRC/1975R, green) and Phalloidin (red).
SDS-PAGE analysis of purified, BSA-free recombinant CD45 antibody (clone PTPRC/1975R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name PTPRC/1975R
Purity Protein A affinity chromatography
UniProt P08575
Localization Cell surface and cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Western blot : 1-2ug/ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant CD45 antibody is available for research use only.
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Description

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Application Notes

Due to variation in protocol and secondary antibody used, the recombinant CD45 antibody may need to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as immunogen for this recombinant CD45 antibody.

Storage

The recombinant CD45 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

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