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Home >> Antibodies >> Recombinant CD45 Antibody / C-Terminal

Recombinant CD45 Antibody / C-Terminal [clone RM291] (R20310)

  Catalog No Formulation Size Price (USD)  
Image R20310-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot testing of human Jurkat cell lysate using recombinant CD45 antibody at a 1:2000 dilution. Expected molecular weight: 147-220 kDa depending on glycosylation level.
IHC staining of formalin fixed and paraffin embedded human tonsil tissue with recombinant CD45 antibody at a 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM291
Purity Protein A purified from animal origin-free supernatant
UniProt P08575
Localization Cell membrane, cytoplasm
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Western Blot : 1:1000-1:2000
Limitations This recombinant CD45 antibody is available for research use only.
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Description

This antibody reacts to human CD45 (Receptor-type tyrosine-protein phosphatase C, PTPRC).

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant CD45 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to the C-terminus of human CD45 (Receptor-type tyrosine-protein phosphatase C) was used as the immunogen for the recombinant CD45 antibody.

Storage

Store the recombinant CD45 antibody at -20oC.

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