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Home >> Antibodies >> Recombinant CD44v6 Antibody

Recombinant CD44v6 Antibody [clone CD44V6/9400R] (V5492)

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  Catalog No Formulation Size Price (USD)  
Image V5492-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5492-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5492SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
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IHC staining of FFPE human esophagus tissue with recombinant CD44v6 antibody (clone CD44V6/9400R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human esophagus tissue with recombinant CD44v6 antibody (clone CD44V6/9400R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD44v6 (CD44V6/9400R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CD44V6/9400R
Purity Protein A/G affinity
UniProt P16070
Localization Cell membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CD44v6 antibody is available for research use only.
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Description

This antibody recognizes an epitope encoded by exon v6 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

Application Notes

Optimal dilution of the recombinant CD44v6 antibody should be determined by the researcher.

Immunogen

A recombinant fragment corresponding to the v3-v10 domain of human CD44 was used as the immunogen for the recombinant CD44v6 antibody.

Storage

Aliquot the recombinant CD44v6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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