• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Recombinant CD4 Antibody

Recombinant CD4 Antibody [clone CD4/3619R] (V8518)

  Catalog No Formulation Size Price (USD)  
Image V8518-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 539
Image
V8518-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 249
Image
V8518SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
Bulk quote request
IHC staining of FFPE human tonsil with recombinant CD4 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node with recombinant CD4 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant CD4 antibody. These results demonstrate the foremost specificity of the CD4/3619R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human tonsil tissue with recombinant CD4 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human tonsil tissue lysate with recombinant CD4 antibody. Expected molecular weight: 50-55 kDa.
Western blot testing of human tonsil tissue lysate with recombinant CD4 antibody. Expected molecular weight: 50-55 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name CD4/3619R
Purity Protein A affinity chromatography
UniProt P01730
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western Blot : 2-4ug/ml
Limitations This recombinant CD4 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : FACS, IF
    Reactivity : Human
    Recmousemono
  • Applications : IHC-P, WB
    Reactivity : Human
    Recmousemono
  • Applications : ELISA
    Reactivity : Human
    Microvalidated
  • Applications : ELISA
    Reactivity : Human
    Microvalidated
  • Applications : IHC-F, FACS
    Reactivity : Human
  • Applications : WB, ELISA
    Reactivity : Human
  • Applications : ELISA, FACS
    Reactivity : Human
  • Applications : Functional studies, FACS, IF
    Reactivity : Human
    Citation  Citations(13)
  • Applications : WB
    Reactivity : Human
    Rab Mono Image
  • Applications : Functional Studies, FACS, IF
    Reactivity : Human
  • Applications : ELISA, Neutralization, FACS, IF
    Reactivity : Mouse
  • Applications : ELISA, FACS
    Reactivity : Human
  • Applications : IHC-P
    Reactivity : Human
  • Applications : WB, FACS, ELISA
    Reactivity : Human
  • Applications : WB, ELISA (peptide)
    Reactivity : Human
  • Applications : WB, IHC-P, FACS, Direct ELISA
    Reactivity : Mouse, Rat

Description

Recombinant CD4 antibody detects CD4, a surface glycoprotein encoded by the CD4 gene. CD4 is expressed on helper T cells, monocytes, macrophages, and dendritic cells, where it functions as a coreceptor for MHC class II-mediated antigen recognition. Because CD4 is critical for T-cell activation and is also the primary receptor for HIV, Recombinant CD4 antibody is indispensable in immunology, oncology, and infectious disease research.

CD4 is a type I transmembrane protein with four extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail containing signaling motifs. By associating with the tyrosine kinase Lck, CD4 stabilizes TCR-MHC interactions and enhances signaling. This costimulatory role supports proliferation, differentiation, and cytokine secretion. Its restricted expression on helper T-cell subsets makes CD4 an important marker for immune monitoring.

The Recombinant CD4 antibody clone CD4/3619R provides reliable and consistent detection. Recombinant technology ensures uniform performance across lots, minimizing variability. Clone CD4/3619R has been referenced in peer-reviewed publications exploring T-cell activation, tumor immunology, and HIV pathogenesis. Its versatility supports flow cytometry, immunohistochemistry, and in vitro functional assays where precise CD4 detection is needed.

Research using clone CD4/3619R has shown how CD4 defines helper T-cell subsets in both health and disease. In oncology, detection supports studies of tumor-infiltrating lymphocytes and anti-tumor immunity. In infectious disease, this antibody has clarified how CD4 serves as the entry receptor for HIV, providing insight into viral pathogenesis and vaccine development. Beyond pathology, CD4 detection is central to studies of immune regulation and tolerance.

NSJ Bioreagents supplies this Recombinant CD4 antibody to support immunology, oncology, and infectious disease research. Alternate designations include T-cell surface glycoprotein CD4 antibody, helper T-cell marker antibody, L3T4 antigen antibody, MHC class II coreceptor antibody, HIV receptor antibody, and CD4 molecule antibody.

Application Notes

Optimal dilution of the recombinant CD4 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 216-396 from the human protein was used as the immunogen for the recombinant CD4 antibody.

Storage

Store the recombinant CD4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.